A Conversation with Christina Smolke

Title : Final report for “ Synthetic RNA controllers for programming mammalian cell fate and function

Informing Biological Design by Integration of Systems and Synthetic Biology

Synthetic biology aims to make the engineering of biology faster and more predictable. In contrast, systems biology focuses on the interaction of myriad components and how these give rise to the dynamic and complex behavior of biological systems. Here, we examine the synergies between these two fields.

Correction for ‘‘Laminin-111 protein therapy prevents muscle disease in the mdx mouse model for Duchenne muscular dys-

Correction for ‘‘Laminin-111 protein therapy prevents muscle disease in the mdx mouse model for Duchenne muscular dystrophy,’’ by Jachinta E. Rooney, Praveen B. Gurpur, and Dean J. Burkin, which appeared in issue 19, May 12, 2009, of Proc Natl Acad Sci USA (106:7991–7996; first published April 28, 2009; 10.1073/pnas.0811599106). The authors note that in preparing Fig. 3A, an image from Fig. 6A ...

Supplemental Data In Vivo Fluorescent Detection of Fe - S Clusters Coordinated by Human GRX 2

Analytical gel filtration A Superdex 75 column driven by an AKTA FPLC system (GE Healthcare) was used to separate monomeric and dimeric Venus-GRX2 immediately prior to spectral measurements and to evaluate the extent to which Venus-Gcn4 had been converted to monomer by enterokinase. All gel filtration experiments involving these proteins were performed using columns that had been equilibrated i...

Higher-order cellular information processing with synthetic RNA devices.

The engineering of biological systems is anticipated to provide effective solutions to challenges that include energy and food production, environmental quality, and health and medicine. Our ability to transmit information to and from living systems, and to process and act on information inside cells, is critical to advancing the scale and complexity at which we can engineer, manipulate, and pr...